THE SERIAL OBSERVATION OF EMBRYOGENESIS IN A CARROT CELL SUSPENSION CULTURE

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Carrot Suspension Culture Cells

Potassium transport in suspension culture cells and protoplasts of carrot.

The properties of potassium transport in carrot (Daucus carota L.) suspension culture cells and their isolated protoplasts were examined. Cells cultured in Murashige and Skoog (MS) medium (Plant Physiol 15: 473-497) were potassium saturated and, consequently, they exhibited little net potassium accumulation. Cells that transport and accumulate potassium were derived from the MS-grown cells by c...

Rapid Effects of IAA on Cell Surface Proteins from Intact Carrot Suspension Culture Cells.

Suspension cultures of carrot (Daucus carrota L.) which had an absolute requirement for exogenously supplied auxin were grown in medium containing indoleacetic acid (IAA) as the sole auxin source. Putative cell surface proteins were extracted from the intact cells. Resupply of IAA to cultures partially depleted of auxin resulted in rapidly increased activities of three enzyme activities subsequ...

Somatic embryogenesis in Phoenix dactylifera: maturation, germination and reduction of hyperhydricity during embryogenic cell suspension culture

This work describes an improved and efficient method for optimum maturation and normal embryo growth to produce healthy plantlets through somatic embryogenesis using suspension cultures for date palm cv. sakkoty. cell suspensions were supplemented with low amounts of dichlorophenoxyacetic acid (0.5mg/1) and different concentrations of abscise acid (AbA) (0.1, 0.3 and 0.5 mg/l) and polyethylene ...